Cost-effective pooling of DNA from nasopharyngeal swab samples for large-scale detection of bacteria by real-time PCR.
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ABSTRACT:
SUBMITTER: Edouard S
PROVIDER: S-EPMC4390636 | biostudies-literature | 2015 Mar
REPOSITORIES: biostudies-literature
Publications
Cost-effective pooling of DNA from nasopharyngeal swab samples for large-scale detection of bacteria by real-time PCR.
Edouard Sophie S Prudent Elsa E Gautret Philippe P Memish Ziad A ZA Raoult Didier D
Journal of clinical microbiology 20141231 3
We investigated the potential of pooling DNA from nasopharyngeal specimens to reduce the cost of real-time PCR (RT-PCR) for bacterial detection. Lyophilization is required to reconcentrate DNA. This strategy yields a high specificity (86%) and a high sensitivity (96%). We estimate that compared to individual testing, 37% fewer RT-PCR tests are needed. ...[more]
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