Ontology highlight
ABSTRACT:
SUBMITTER: Broccardo CJ
PROVIDER: S-EPMC4391816 | biostudies-literature | 2013 Sep
REPOSITORIES: biostudies-literature
Broccardo Carolyn J CJ Schauer Kevin L KL Kohrt Wendy M WM Schwartz Robert S RS Murphy James P JP Prenni Jessica E JE
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 20130704
A novel microfluidic chromatography device coupled with tandem mass spectrometry (LC-MS/MS) was utilized for the multiplex analysis of 5 steroids (testosterone, dihydrotestosterone, progesterone, cortisol, cortisone) in human serum. The use of microfluidics allowed for reduction of the chromatographic flow rate to 3μl/min with overall method run times comparable to standard flow LC-MS/MS methods reported in the literature, corresponding to a 150 fold decrease in solvent consumption. Furthermore, ...[more]