Ontology highlight
ABSTRACT:
SUBMITTER: Keerti
PROVIDER: S-EPMC4392994 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Keerti Gupta Anuradha A Kumar Vinod V Dubey Ashutosh A Verma A K AK
ISRN biochemistry 20140220
A thermostable β-glucosidase was effectively immobilized on alginate by the method of gel entrapment. After optimization of immobilized conditions, recovered enzyme activity was 60%. Optimum pH, temperature, kinetic parameters, thermal and pH stability, reusability, and storage stability were investigated. The K m and V max for immobilized β-glucosidase were estimated to be 5.0 mM and 0.64 U/ml, respectively. When comparing, free and immobilized enzyme, change was observed in optimum pH and temp ...[more]