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Transcriptome microRNA profiling of bovine mammary glands infected with Staphylococcus aureus.


ABSTRACT: MicroRNAs are small non-coding RNA molecules that are important regulators of gene expression at the post-transcriptional level. miRNAs impact the processes of cell proliferation, differentiation and apoptosis. Thus, the regulation of miRNA expression profiles associated with mastitis will be conducive for its control. In this study, Staphylococcus aureus (S. aureus) was administered to the mammary gland of Chinese Holstein cows to construct a bacteria-type mastitis model. Total RNA was isolated from bovine mammary gland tissue samples from the S. aureus-induced mastitis group and controls. miRNAs were analyzed using Solexa sequencing and bioinformatics processing for the experimental group and control group. Two miRNA libraries were constructed respectively. A total of 370 known bovine miRNAs and 341 novel mi RNAs were detected for the S. aureus and 358 known bovine miRNAs and 232 novel miRNAs for control groups. A total of 77 miRNAs in the S. aureus group showed significant differences compared to the control group. GO (Gene Ontology) analysis showed these target genes were involved in the regulation of cells, binding, etc., while KEGG (Kyoto Encyclopedia of Genes and Genomes) analysis showed that these genes were enriched in endocytosis, and olfactory transduction pathways involved in cancer. These results provide an experimental basis to reveal the cause and regulatory mechanism of mastitis and also suggest the potential of miRNAs to serve as biomarkers for the diagnosis of mastitis in dairy cows.

SUBMITTER: Li R 

PROVIDER: S-EPMC4394461 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature

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Transcriptome microRNA profiling of bovine mammary glands infected with Staphylococcus aureus.

Li Rui R   Zhang Cheng-Long CL   Liao Xiang-Xiang XX   Chen Dan D   Wang Wen-Qiang WQ   Zhu Yi-Hui YH   Geng Xiao-Han XH   Ji De-Jun DJ   Mao Yong-Jiang YJ   Gong Yun-Chen YC   Yang Zhang-Ping ZP  

International journal of molecular sciences 20150304 3


MicroRNAs are small non-coding RNA molecules that are important regulators of gene expression at the post-transcriptional level. miRNAs impact the processes of cell proliferation, differentiation and apoptosis. Thus, the regulation of miRNA expression profiles associated with mastitis will be conducive for its control. In this study, Staphylococcus aureus (S. aureus) was administered to the mammary gland of Chinese Holstein cows to construct a bacteria-type mastitis model. Total RNA was isolated  ...[more]

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