Spectrofluorometric and molecular docking studies on the binding of curcumenol and curcumenone to human serum albumin.

ABSTRACT: Curcumenol and curcumenone are two major constituents of the plants of medicinally important genus of Curcuma, and often govern the pharmacological effect of these plant extracts. These two compounds, isolated from C. zedoaria rhizomes were studied for their binding to human serum albumin (HSA) using the fluorescence quench titration method. Molecular docking was also performed to get a more detailed insight into their interaction with HSA at the binding site. Additions of these sesquiterpenes to HSA produced significant fluorescence quenching and blue shifts in the emission spectra of HSA. Analysis of the fluorescence data pointed toward moderate binding affinity between the ligands and HSA, with curcumenone showing a relatively higher binding constant (2.46 × 105 M-1) in comparison to cu

SUBMITTER: Hamdi OA 

PROVIDER: S-EPMC4394470 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature