Ontology highlight
ABSTRACT:
SUBMITTER: Li C
PROVIDER: S-EPMC4395411 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Li Cheng C Wu Alex Chi AC Go Rob Marc RM Malouf Jacob J Turner Mark S MS Malde Alpeshkumar K AK Mark Alan E AE Gilbert Robert G RG
PloS one 20150413 4
Starch is a complex branched glucose polymer whose branch molecular weight distribution (the chain-length distribution, CLD) influences nutritionally important properties such as digestion rate. Chain-stopping in starch biosynthesis is by starch branching enzyme (SBE). Site-directed mutagenesis was used to modify SBEIIa from Zea mays (mSBEIIa) to produce mutants, each differing in a single conserved amino-acid residue. Products at different times from in vitro branching were debranched and the t ...[more]