Simple multiplex PCR assays to detect common pathogens and associated genes encoding for acquired extended spectrum betalactamases (ESBL) or carbapenemases from surgical site specimens in Vietnam.

ABSTRACT:

Unlabelled

Surgical site infection (SSI) is common in Vietnamese post-operative patients. It contributes to increased morbidity, mortality, hospitalization time and health care expenditure. Bacterial culture is considered the gold standard procedure to identify SSI pathogens and antibiotic resistant properties; however, it can detect microbes that can readily grow and is time-consuming. We propose optimized multiplex PCR assays to diagnose the most relevant microbes and associated genes encoding for acquired extended spectrum betalactamases (ESBL) or carbapenemases from Vietnamese patients with SSI in a hospital setting in Hanoi.

Methods

Ninety-one patients (n = 91) were collected in order to identify microbial pathogens and associated genes encoding for acquired extended sp

SUBMITTER: Trung NT 

PROVIDER: S-EPMC4399146 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature