Dataset Information

Detection of enterovirus D68 in Canadian laboratories.

ABSTRACT: The recent emergence of a severe respiratory disease caused by enterovirus D68 prompted investigation into whether Canadian hospital and provincial laboratories can detect this virus using commercial and laboratory-developed assays. This study demonstrated analytical sensitivity differences between commercial and laboratory-developed assays for the detection of enterovirus D68.

SUBMITTER: Hatchette TF

PROVIDER: S-EPMC4400745 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature

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Detection of enterovirus D68 in Canadian laboratories.

Hatchette Todd F TF Drews Steven J SJ Grudeski Elsie E Booth Tim T Martineau Christine C Dust Kerry K Garceau Richard R Gubbay Jonathan J Karnauchow Tim T Krajden Mel M Levett Paul N PN Mazzulli Tony T McDonald Ryan R RR McNabb Alan A Mubareka Samira S Needle Robert R Petrich Astrid A Richardson Susan S Rutherford Candy C Smieja Marek M Tellier Raymond R Tipples Graham G LeBlanc Jason J JJ

Journal of clinical microbiology 20150304 5

The recent emergence of a severe respiratory disease caused by enterovirus D68 prompted investigation into whether Canadian hospital and provincial laboratories can detect this virus using commercial and laboratory-developed assays. This study demonstrated analytical sensitivity differences between commercial and laboratory-developed assays for the detection of enterovirus D68. ...[more]

PMID: 25740765

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Project description:BackgroundEnterovirus D68 (EV-D68) is a re-emerging pathogen that is particularly common in children and may cause asthma-like respiratory infection and acute flaccid myelitis. However, in Korea, EV-D68 has never been reported thus far. This study aimed to identify EV-D68 from nasopharyngeal aspirates (NPAs) in Korean children with a respiratory tract infection.Materials and methodsThe EV-D68 reference strain was purchased and blindly used to assess the detection ability of three commercial and one in-house mRT-PCR kit in 2018. Then, we selected children whose specimens were positive for human rhinovirus (HRV) and/or enterovirus (EV) by Allplex mRT-PCR (Seegene, Inc., Seoul, Korea) from April to December 2022. Total RNA was extracted from NPAs, and a partial 5'-UTR gene was amplified and sequenced for the identification of HRV/EV species. Additionally, PCR targeting the VP1 gene was performed to assess EV-D68-positive NPAs, followed by sequencing. Phylogenetic analysis and comparison of amino acid sequence alignments were performed using a partial VP1 gene of our and recent international EV-D68 strains.ResultsAmong the mRT-PCR kits tested, only the in-house kit was able to detect EV-D68 in 2018. However, we detected three EV-D68 strains among children hospitalized with fever and/or respiratory symptoms in September - December 2022 who tested positive for EV by the Allplex kit. Two of them were healthy toddlers with lower respiratory infections accompanied by new-onset wheezing but no neurologic complications. Among 34 children with lower respiratory infection who tested positive for HRV during the same period, EV-D68 was not detected. Phylogenetic analysis revealed that the first Korean EV-D68 belonged to subclade B3. Amino acid sequence alignment of international subclade B3 EV-D68 strains also showed that our strain is genetically more related to those from Europe than those from Japan.ConclusionWe first detected EV-D68 in three Korean children who had EV detected by the Allplex mRT-PCR kit in 2022. EV-D68 also circulated in Korea in fall 2022, but the prevalence and severity seemed to be lower than those in previous reports from other countries.

Dataset Information

Detection of enterovirus D68 in Canadian laboratories.

Publications

Detection of enterovirus D68 in Canadian laboratories.

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