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Comparison of Abbott RealTime HCV Genotype II with Versant line probe assay 2.0 for hepatitis C virus genotyping.


ABSTRACT: Genotyping and subtyping of 225 samples with hepatitis C virus (HCV) genotype 1, 2, 3, or 6 infection were done with Versant LiPA 2.0 and Abbott RealTime HCV Genotype (GT) II by using direct sequencing of the NS5B and 5' untranslated regions as the reference standards. The concordance rates were >99.2% for genotypes and 96.1% for subtypes 1a and 1b. Both the Abbott RealTime and Versant LiPA assays can accurately determine hepatitis C virus genotypes. (This study has been registered at ClinicalTrials.gov under registration no. NCT00979979.).

SUBMITTER: Liu CH 

PROVIDER: S-EPMC4400777 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature

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Comparison of Abbott RealTime HCV Genotype II with Versant line probe assay 2.0 for hepatitis C virus genotyping.

Liu Chen-Hua CH   Liang Cheng-Chao CC   Liu Chun-Jen CJ   Lin Chih-Lin CL   Su Tung-Hung TH   Yang Hung-Chih HC   Chen Pei-Jer PJ   Chen Ding-Shinn DS   Kao Jia-Horng JH  

Journal of clinical microbiology 20150304 5


Genotyping and subtyping of 225 samples with hepatitis C virus (HCV) genotype 1, 2, 3, or 6 infection were done with Versant LiPA 2.0 and Abbott RealTime HCV Genotype (GT) II by using direct sequencing of the NS5B and 5' untranslated regions as the reference standards. The concordance rates were >99.2% for genotypes and 96.1% for subtypes 1a and 1b. Both the Abbott RealTime and Versant LiPA assays can accurately determine hepatitis C virus genotypes. (This study has been registered at ClinicalTr  ...[more]

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