Biofuels. Engineering alcohol tolerance in yeast.
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ABSTRACT:
SUBMITTER: Lam FH
PROVIDER: S-EPMC4401034 | biostudies-literature | 2014 Oct
REPOSITORIES: biostudies-literature
Publications
Biofuels. Engineering alcohol tolerance in yeast.
Lam Felix H FH Ghaderi Adel A Fink Gerald R GR Stephanopoulos Gregory G
Science (New York, N.Y.) 20141002 6205
Ethanol toxicity in the yeast Saccharomyces cerevisiae limits titer and productivity in the industrial production of transportation bioethanol. We show that strengthening the opposing potassium and proton electrochemical membrane gradients is a mechanism that enhances general resistance to multiple alcohols. The elevation of extracellular potassium and pH physically bolsters these gradients, increasing tolerance to higher alcohols and ethanol fermentation in commercial and laboratory strains (in ...[more]
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