Acute targeting of general transcription factor IIB restricts cardiac hypertrophy via selective inhibition of gene transcription.

ABSTRACT:

Background

We previously reported that specialized and housekeeping genes are differentially regulated via de novo recruitment and pause-release of RNA polymerase II, respectively, during cardiac hypertrophy. However, the significance of this finding remains to be examined. Therefore, the purpose of this study was to determine the mechanisms that differentially regulate these gene groups and exploit them for therapeutic targeting.

Methods and results

Here, we show that general transcription factor IIB (TFIIB) and cyclin-dependent kinase 9 are upregulated during hypertrophy, both targeted by microRNA-1, and play preferential roles in regulating those 2 groups of genes. Chromatin immunoprecipitation-sequencing reveals that TFIIB is constitutively bound to all paused, housekeep

SUBMITTER: Sayed D 

PROVIDER: S-EPMC4401077 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature