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Structural insights into the role of rRNA modifications in protein synthesis and ribosome assembly.

ABSTRACT: We report crystal structures of the Thermus thermophilus ribosome at 2.3- to 2.5-Å resolution, which have enabled modeling of rRNA modifications. The structures reveal contacts of modified nucleotides with mRNA and tRNAs or protein pY, and contacts within the ribosome interior stabilizing the functional fold of rRNA. Our work provides a resource to explore the roles of rRNA modifications and yields a more comprehensive atomic model of a bacterial ribosome.

SUBMITTER: Polikanov YS

PROVIDER: S-EPMC4401423 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature

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Structural insights into the role of rRNA modifications in protein synthesis and ribosome assembly.

Polikanov Yury S YS Melnikov Sergey V SV Söll Dieter D Steitz Thomas A TA

Nature structural & molecular biology 20150316 4

We report crystal structures of the Thermus thermophilus ribosome at 2.3- to 2.5-Å resolution, which have enabled modeling of rRNA modifications. The structures reveal contacts of modified nucleotides with mRNA and tRNAs or protein pY, and contacts within the ribosome interior stabilizing the functional fold of rRNA. Our work provides a resource to explore the roles of rRNA modifications and yields a more comprehensive atomic model of a bacterial ribosome. ...[more]

PMID: 25775268

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