RNA chaperones buffer deleterious mutations in E. coli.
Ontology highlight
ABSTRACT:
SUBMITTER: Rudan M
PROVIDER: S-EPMC4402597 | biostudies-literature | 2015 Mar
REPOSITORIES: biostudies-literature
Publications
RNA chaperones buffer deleterious mutations in E. coli.
Rudan Marina M Schneider Dominique D Warnecke Tobias T Krisko Anita A
eLife 20150325
Both proteins and RNAs can misfold into non-functional conformations. Protein chaperones promote native folding of nascent polypeptides and refolding of misfolded species, thereby buffering mutations that compromise protein structure and function. Here, we show that RNA chaperones can also act as mutation buffers that enhance organismal fitness. Using competition assays, we demonstrate that overexpression of select RNA chaperones, including three DEAD box RNA helicases (DBRHs) (CsdA, SrmB, RhlB) ...[more]
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