Dataset Information

A new model to study sleep deprivation-induced seizure.

ABSTRACT:

Background and study objectives

A relationship between sleep and seizures is well-described in both humans and rodent animal models; however, the mechanism underlying this relationship is unknown. Using Drosophila melanogaster mutants with seizure phenotypes, we demonstrate that seizure activity can be modified by sleep deprivation.

Design

Seizure activity was evaluated in an adult bang-sensitive seizure mutant, stress sensitive B (sesB(9ed4)), and in an adult temperature sensitive seizure mutant seizure (sei(ts1)) under baseline and following 12 h of sleep deprivation. The long-term effect of sleep deprivation on young, immature sesB(9ed4) flies was also assessed.

Setting

Laboratory.

Participants

Drosophila melanogaster.

Interventions

Sleep deprivation.

Measurements and results

Sleep deprivation increased seizure susceptibility in adult sesB(9ed4)/+ and sei(ts1) mutant flies. Sleep deprivation also increased seizure susceptibility when sesB was disrupted using RNAi. The effect of sleep deprivation on seizure activity was reduced when sesB(9ed4)/+ flies were given the anti-seizure drug, valproic acid. In contrast to adult flies, sleep deprivation during early fly development resulted in chronic seizure susceptibility when sesB(9ed4)/+ became adults.

Conclusions

These findings show that Drosophila is a model organism for investigating the relationship between sleep and seizure activity.

SUBMITTER: Lucey BP

PROVIDER: S-EPMC4402675 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

A new model to study sleep deprivation-induced seizure.

Lucey Brendan P BP Leahy Averi A Rosas Regine R Shaw Paul J PJ

Sleep 20150501 5

<h4>Background and study objectives</h4>A relationship between sleep and seizures is well-described in both humans and rodent animal models; however, the mechanism underlying this relationship is unknown. Using Drosophila melanogaster mutants with seizure phenotypes, we demonstrate that seizure activity can be modified by sleep deprivation.<h4>Design</h4>Seizure activity was evaluated in an adult bang-sensitive seizure mutant, stress sensitive B (sesB(9ed4)), and in an adult temperature sensitiv ...[more]

PMID: 25515102

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Dataset Information

A new model to study sleep deprivation-induced seizure.

Background and study objectives

Design

Setting

Participants

Interventions

Measurements and results

Conclusions

Publications

A new model to study sleep deprivation-induced seizure.

Similar Datasets

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