Ontology highlight
ABSTRACT:
SUBMITTER: Lee YT
PROVIDER: S-EPMC4403241 | biostudies-literature | 2015 Jun
REPOSITORIES: biostudies-literature
Lee Young-Tae YT Gibbons Garrett G Lee Shirley Y SY Nikolovska-Coleska Zaneta Z Dou Yali Y
Protein expression and purification 20150214
We report an optimized method to purify and reconstitute histone octamer, which utilizes high expression of histones in inclusion bodies but eliminates the time consuming steps of individual histone purification. In the newly modified protocol, Xenopus laevis H2A, H2B, H3, and H4 are expressed individually into inclusion bodies of bacteria, which are subsequently mixed together and denatured in 8M guanidine hydrochloride. Histones are refolded and reconstituted into soluble octamer by dialysis a ...[more]