A novel calb-type lipase discovered by fungal genomes mining.
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ABSTRACT:
SUBMITTER: Vaquero ME
PROVIDER: S-EPMC4405274 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Publications
A novel calb-type lipase discovered by fungal genomes mining.
Vaquero Maria E ME de Eugenio Laura I LI Martínez Maria J MJ Barriuso Jorge J
PloS one 20150421 4
The fungus Pseudozyma antarctica produces a lipase (CalB) with broad substrate specificity, stability, high regio- and enantio-selectivity. It is active in non-aqueous organic solvents and at elevated temperatures. Hence, CalB is a robust biocatalyst for chemical conversions on an industrial scale. Here we report the in silico mining of public metagenomes and fungal genomes to discover novel lipases with high homology to CalB. The candidates were selected taking into account homology and conserv ...[more]
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