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Bio301: A Web-Based EST Annotation Pipeline That Facilitates Functional Comparison Studies.


ABSTRACT: In this postgenomic era, a huge volume of information derived from expressed sequence tags (ESTs) has been constructed for functional description of gene expression profiles. Comparative studies have become more and more important to researchers of biology. In order to facilitate these comparative studies, we have constructed a user-friendly EST annotation pipeline with comparison tools on an integrated EST service website, Bio301. Bio301 includes regular EST preprocessing, BLAST similarity search, gene ontology (GO) annotation, statistics reporting, a graphical GO browsing interface, and microarray probe selection tools. In addition, Bio301 is equipped with statistical library comparison functions using multiple EST libraries based on GO annotations for mining meaningful biological information.

SUBMITTER: Chen YC 

PROVIDER: S-EPMC4407203 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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Bio301: A Web-Based EST Annotation Pipeline That Facilitates Functional Comparison Studies.

Chen Yen-Chen YC   Chen Yun-Ching YC   Lin Wen-Dar WD   Hsiao Chung-Der CD   Chiu Hung-Wen HW   Ho Jan-Ming JM  

ISRN bioinformatics 20110927


In this postgenomic era, a huge volume of information derived from expressed sequence tags (ESTs) has been constructed for functional description of gene expression profiles. Comparative studies have become more and more important to researchers of biology. In order to facilitate these comparative studies, we have constructed a user-friendly EST annotation pipeline with comparison tools on an integrated EST service website, Bio301. Bio301 includes regular EST preprocessing, BLAST similarity sear  ...[more]

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