Haplotype analysis of the Apolipoprotein A5 gene in Moroccan patients with the metabolic syndrome.

ABSTRACT: BACKGROUND:In this case-control study we investigated the relative contribution of commons APOA5 polymorphisms and haplotypes to the risk of metabolic syndrome in Moroccan patients. METHODS:Using the International Diabetes Federation (IDF) criteria for metabolic syndrome, the study included 176 patients and 105 controls. We genotyped APOA5 polymorphisms (-1131 T?>?C, c.56C?>?G, c.553G?>?T and c.1259 T?>?C) by PCR-RFLP analysis. The effects of APOA5 polymorphisms and constructed haplotypes on metabolic syndrome were estimated using logistic regression analyses. RESULTS:The statistical analysis showed a significant association between APOA5 -1131 T?>?C and APOA5 c.56C?>?G polymorphisms with metabolic syndrome in both Codominant and Dominant models. The APOA5 -1131 T?>?C polymorphism was associated with increased fasting glucose (p?=?0.0295) and reduced HDL levels (p?=?0.0091). Carriers of the APOA5 c.56G allele had increased triglyceride levels (p?=?0.0435) and waist circumference (p?=?0.0122). Similarly the APOA5 1259 T?>?C variant was associated with increased waist circumference (p?=?0.0463). The haplotypes CCGT (OR?=?3.223; p?=?0.00278) and CGGT (OR?=?8.234; p?=?0.00534) were significantly associated with susceptibility to metabolic syndrome. CONCLUSIONS:Our results confirms the association of APOA5 -1131 T?>?C and c.56C?>?G variants with the predisposition to metabolic syndrome complications.

SUBMITTER: Ajjemami M 

PROVIDER: S-EPMC4407873 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature