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Electronic and structural elements that regulate the excited-state dynamics in purine nucleobase derivatives.

ABSTRACT: The excited-state dynamics of the purine free base and 9-methylpurine are investigated using experimental and theoretical methods. Femtosecond broadband transient absorption experiments reveal that excitation of these purine derivatives in aqueous solution at 266 nm results primarily in ultrafast conversion of the S2(??*) state to the vibrationally excited (1)n?* state. Following vibrational and conformational relaxation, the (1)n?* state acts as a doorway state in the efficient population of the triplet manifold with an intersystem crossing lifetime of hundreds of picoseconds. Experiments show an almost 2-fold increase in the intersystem crossing rate on going from polar aprotic to nonpolar solvents, suggesting that a solvent-dependent energy barrier must be surmounted to access the singlet-to-triplet crossing region. Ab initio static and surface-hopping dynamics simulations lend strong support to the proposed relaxation mechanism. Collectively, the experimental and computational results demonstrate that the accessibility of the n?* states and the topology of the potential energy surfaces in the vicinity of conical intersections are key elements in controlling the excited-state dynamics of the purine derivatives. From a structural perspective, it is shown that the purine chromophore is not responsible for the ultrafast internal conversion in the adenine and guanine monomers. Instead, C6 functionalization plays an important role in regulating the rates of radiative and nonradiative relaxation. C6 functionalization inhibits access to the (1)n?* state while simultaneously facilitating access to the (1)??*(La)/S0 conical intersection, such that population of the (1)n?* state cannot compete with the relaxation pathways to the ground state involving ring puckering at the C2 position.

SUBMITTER: Crespo-Hernandez CE

PROVIDER: S-EPMC4410903 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature

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Electronic and structural elements that regulate the excited-state dynamics in purine nucleobase derivatives.

Crespo-Hernández Carlos E CE Martínez-Fernández Lara L Rauer Clemens C Reichardt Christian C Mai Sebastian S Pollum Marvin M Marquetand Philipp P González Leticia L Corral Inés I

Journal of the American Chemical Society 20150325 13

The excited-state dynamics of the purine free base and 9-methylpurine are investigated using experimental and theoretical methods. Femtosecond broadband transient absorption experiments reveal that excitation of these purine derivatives in aqueous solution at 266 nm results primarily in ultrafast conversion of the S2(ππ*) state to the vibrationally excited (1)nπ* state. Following vibrational and conformational relaxation, the (1)nπ* state acts as a doorway state in the efficient population of th ...[more]

PMID: 25763596

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Electronic and structural elements that regulate the excited-state dynamics in purine nucleobase derivatives.

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Electronic and structural elements that regulate the excited-state dynamics in purine nucleobase derivatives.

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