Ontology highlight
ABSTRACT:
SUBMITTER: Polstein LR
PROVIDER: S-EPMC4412021 | biostudies-literature | 2015 Mar
REPOSITORIES: biostudies-literature
Polstein Lauren R LR Gersbach Charles A CA
Nature chemical biology 20150209 3
Optogenetic systems enable precise spatial and temporal control of cell behavior. We engineered a light-activated CRISPR-Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light. This was accomplished by fusing the light-inducible heterodimerizing proteins CRY2 and CIB1 to a transactivation domain and the catalytically inactive dCas9, respectively. The versatile LACE system can be easily directed to new DNA sequences for the dynamic regulation of e ...[more]