Global and quantitative profiling of polyadenylated RNAs using PAS-seq.
Ontology highlight
ABSTRACT:
SUBMITTER: Yao C
PROVIDER: S-EPMC4412162 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Publications
Global and quantitative profiling of polyadenylated RNAs using PAS-seq.
Yao Chengguo C Shi Yongsheng Y
Methods in molecular biology (Clifton, N.J.) 20140101
mRNA alternative polyadenylation (APA) has been increasingly recognized as a widespread and evolutionarily conserved mechanism for eukaryotic gene regulation. Here we describe a method called poly(A) site sequencing that can not only map RNA polyadenylation sites on a transcriptome level but also provide quantitative information on the relative abundance of polyadenylated RNAs. This method has been successfully used for both global APA analysis and digital gene expression profiling. ...[more]
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