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Genotype to phenotype: a complex problem.

ABSTRACT: We generated a high-resolution whole-genome sequence and individually deleted 5100 genes in Sigma1278b, a Saccharomyces cerevisiae strain closely related to reference strain S288c. Similar to the variation between human individuals, Sigma1278b and S288c average 3.2 single-nucleotide polymorphisms per kilobase. A genome-wide comparison of deletion mutant phenotypes identified a subset of genes that were conditionally essential by strain, including 44 essential genes unique to Sigma1278b and 13 unique to S288c. Genetic analysis indicates the conditional phenotype was most often governed by complex genetic interactions, depending on multiple background-specific modifiers. Our comprehensive analysis suggests that the presence of a complex set of modifiers will often underlie the phenotypic differences between individuals.

SUBMITTER: Dowell RD

PROVIDER: S-EPMC4412269 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature

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Genotype to phenotype: a complex problem.

Dowell Robin D RD Ryan Owen O Jansen An A Cheung Doris D Agarwala Sudeep S Danford Timothy T Bernstein Douglas A DA Rolfe P Alexander PA Heisler Lawrence E LE Chin Brian B Nislow Corey C Giaever Guri G Phillips Patrick C PC Fink Gerald R GR Gifford David K DK Boone Charles C

Science (New York, N.Y.) 20100401 5977

We generated a high-resolution whole-genome sequence and individually deleted 5100 genes in Sigma1278b, a Saccharomyces cerevisiae strain closely related to reference strain S288c. Similar to the variation between human individuals, Sigma1278b and S288c average 3.2 single-nucleotide polymorphisms per kilobase. A genome-wide comparison of deletion mutant phenotypes identified a subset of genes that were conditionally essential by strain, including 44 essential genes unique to Sigma1278b and 13 un ...[more]

PMID: 20413493

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