Direct conversion of human myoblasts into brown-like adipocytes by engineered super-active PPAR?.

ABSTRACT: To determine whether super-activation of PPAR? can reprogram human myoblasts into brown-like adipocytes and to establish a new cell model for browning research.To enhance the PPAR? signaling, M3, the transactivation domain of MyoD, was fused to PPAR?. PPAR? and M3-PPAR?-lentiviral vectors were used to convert human myoblasts into adipocytes. Brown adipocyte markers of the reprogrammed adipocytes were assessed by qPCR and protein analyses. White adipocytes differentiated from subcutaneous stromal vascular cells and perithyroid brown fat tissues were used as references.In transient transfections, M3-PPAR? had a stronger constitutive activity than PPAR? by reporter assay. Although the transduction of either PPAR? or M3-PPAR? induced adipogenesis in myoblasts, M3-PPAR? drastically induced the brown adipocyte markers of UCP1, CIDEA, and PRDM16 by 1,050, 2.4, and 5.0 fold, respectively and increased mitochondria contents by 4 fold, compared to PPAR?.Super-activation of PPAR? can effectively convert human myoblasts into brown-like adipocytes and a new approach to derive brown-like adipocytes.

SUBMITTER: Zhu Y 

PROVIDER: S-EPMC4413469 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature