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Direct visualization of newly synthesized target proteins in situ.

ABSTRACT: Protein synthesis is a dynamic process that tunes the cellular proteome in response to internal and external demands. Metabolic labeling approaches identify the general proteomic response but cannot visualize specific newly synthesized proteins within cells. Here we describe a technique that couples noncanonical amino acid tagging or puromycylation with the proximity ligation assay to visualize specific newly synthesized proteins and monitor their origin, redistribution and turnover in situ.

SUBMITTER: tom Dieck S

PROVIDER: S-EPMC4414919 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature

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Direct visualization of newly synthesized target proteins in situ.

tom Dieck Susanne S Kochen Lisa L Hanus Cyril C Heumüller Maximilian M Bartnik Ina I Nassim-Assir Belquis B Merk Katrin K Mosler Thorsten T Garg Sakshi S Bunse Stefanie S Tirrell David A DA Schuman Erin M EM

Nature methods 20150316 5

Protein synthesis is a dynamic process that tunes the cellular proteome in response to internal and external demands. Metabolic labeling approaches identify the general proteomic response but cannot visualize specific newly synthesized proteins within cells. Here we describe a technique that couples noncanonical amino acid tagging or puromycylation with the proximity ligation assay to visualize specific newly synthesized proteins and monitor their origin, redistribution and turnover in situ. ...[more]

PMID: 25775042

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