Unknown

Dataset Information

0

Double-strand breaks from a radical commonly produced by DNA-damaging agents.


ABSTRACT: Double-strand breaks are widely accepted to be the most toxic form of DNA damage. Molecules that produce double-strand breaks via a single chemical event are typically very cytotoxic and far less common than those that form single-strand breaks. It was recently reported that a commonly formed C4'-radical produces double-strand breaks under aerobic conditions. Experiments described herein indicate that a peroxyl radical initiates strand damage on the complementary strand via C4'-hydrogen atom abstraction. Inferential evidence suggests that a C3'-peroxyl radical induces complementary strand damage more efficiently than does a C4'-peroxyl radical. Complementary strand hydrogen atom abstraction by the peroxyl radical is efficiently quenched by thiols. This mechanism could contribute to the higher than expected yield of double-strand breaks produced by ionizing radiation.

SUBMITTER: Taverna Porro ML 

PROVIDER: S-EPMC4415041 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature

altmetric image

Publications

Double-strand breaks from a radical commonly produced by DNA-damaging agents.

Taverna Porro Marisa L ML   Greenberg Marc M MM  

Chemical research in toxicology 20150309 4


Double-strand breaks are widely accepted to be the most toxic form of DNA damage. Molecules that produce double-strand breaks via a single chemical event are typically very cytotoxic and far less common than those that form single-strand breaks. It was recently reported that a commonly formed C4'-radical produces double-strand breaks under aerobic conditions. Experiments described herein indicate that a peroxyl radical initiates strand damage on the complementary strand via C4'-hydrogen atom abs  ...[more]

Similar Datasets

| S-EPMC133806 | biostudies-literature
| S-EPMC5664317 | biostudies-other
| S-EPMC4861929 | biostudies-literature
| S-EPMC7379675 | biostudies-literature
| S-EPMC5577458 | biostudies-literature
| S-EPMC2779242 | biostudies-literature
| S-EPMC5409072 | biostudies-literature
| S-EPMC2246615 | biostudies-literature
| S-EPMC4826256 | biostudies-literature
| S-EPMC5714177 | biostudies-literature