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Developing a common bean core collection suitable for association mapping studies.

ABSTRACT: Because of the continuous introduction of germplasm from abroad, some collections have a high number of accessions, making it difficult to explore the genetic variability present in a germplasm bank for conservation and breeding purposes. Therefore, the aim of this study was to quantify and analyze the structure of genetic variability among 500 common bean accessions to construct a core collection. A total of 58 SSRs were used for this purpose. The polymorphism information content (PIC) in the 180 common bean accessions selected to compose the core collection ranged from 0.17 to 0.86, and the discriminatory power (DP) ranged from 0.21 to 0.90. The 500 accessions were clustered into 15 distinct groups and the 180 accessions into four distinct groups in the Structure analysis. According to analysis of molecular variance, the most divergent accessions comprised 97.2% of the observed genetic variability present within the base collection, confirming the efficiency of the selection criterion. The 180 selected accessions will be used for association mapping in future studies and could be potentially used by breeders to direct new crosses and generate elite cultivars that meet current and future global market needs.

SUBMITTER: Perseguini JM

PROVIDER: S-EPMC4415564 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature

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Developing a common bean core collection suitable for association mapping studies.

Perseguini Juliana Morini Küpper Cardoso JM Silva Gliciane Micaele Borges GM Rosa João Ricardo Bachega Feijó JR Gazaffi Rodrigo R Marçal Jéssica Fernanda JF Carbonell Sérgio Augusto Morais SA Chiorato Alisson Fernando AF Zucchi Maria Imaculada MI Garcia Antonio Augusto Franco AA Benchimol-Reis Luciana Lasry LL

Genetics and molecular biology 20140317 1

Because of the continuous introduction of germplasm from abroad, some collections have a high number of accessions, making it difficult to explore the genetic variability present in a germplasm bank for conservation and breeding purposes. Therefore, the aim of this study was to quantify and analyze the structure of genetic variability among 500 common bean accessions to construct a core collection. A total of 58 SSRs were used for this purpose. The polymorphism information content (PIC) in the 1 ...[more]

PMID: 25983627

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Developing a common bean core collection suitable for association mapping studies.

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Developing a common bean core collection suitable for association mapping studies.

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