UV-CD12: synchrotron radiation circular dichroism beamline at ANKA.

ABSTRACT: Synchrotron radiation circular dichroism (SRCD) is a rapidly growing technique for structure analysis of proteins and other chiral biomaterials. UV-CD12 is a high-flux SRCD beamline installed at the ANKA synchrotron, to which it had been transferred after the closure of the SRS Daresbury. The beamline covers an extended vacuum-UV to near-UV spectral range and has been open for users since October 2011. The current end-station allows for temperature-controlled steady-state SRCD spectroscopy, including routine automated thermal scans of microlitre volumes of water-soluble proteins down to 170?nm. It offers an excellent signal-to-noise ratio over the whole accessible spectral range. The technique of oriented circular dichroism (OCD) was recently implemented for determining the membrane alignment of ?-helical peptides and proteins in macroscopically oriented lipid bilayers as mimics of cellular membranes. It offers improved spectral quality <200?nm compared with an OCD setup adapted to a bench-top instrument, and accelerated data collection by a factor of ?3. In addition, it permits investigations of low hydrated protein films down to 130?nm using a rotatable sample cell that avoids linear dichroism artifacts.

SUBMITTER: Burck J 

PROVIDER: S-EPMC4416691 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature