Electric LAMP: Virtual Loop-Mediated Isothermal AMPlification.
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ABSTRACT:
SUBMITTER: Salinas NR
PROVIDER: S-EPMC4417551 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Publications
Electric LAMP: Virtual Loop-Mediated Isothermal AMPlification.
Salinas Nelson R NR Little Damon P DP
ISRN bioinformatics 20121121
We present eLAMP, a PERL script, with Tk graphical interface, that electronically simulates Loop-mediated AMPlification (LAMP) allowing users to efficiently test putative LAMP primers on a set of target sequences. eLAMP can match primers to templates using either exact (via builtin PERL regular expressions) or approximate matching (via the tre-agrep library). Performance was tested on 40 whole genome sequences of Staphylococcus. eLAMP correctly predicted that the two tested primer sets would amp ...[more]
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