Ontology highlight
ABSTRACT:
SUBMITTER: Parnell TJ
PROVIDER: S-EPMC4423118 | biostudies-literature | 2015 Mar
REPOSITORIES: biostudies-literature
Parnell Timothy J TJ Schlichter Alisha A Wilson Boris G BG Cairns Bradley R BR
eLife 20150330
ISWI family chromatin remodelers typically organize nucleosome arrays, while SWI/SNF family remodelers (RSC) typically disorganize and eject nucleosomes, implying an antagonism that is largely unexplored in vivo. Here, we describe two independent genetic screens for rsc suppressors that yielded mutations in the promoter-focused ISW1a complex or mutations in the 'basic patch' of histone H4 (an epitope that regulates ISWI activity), strongly supporting RSC-ISW1a antagonism in vivo. RSC and ISW1a l ...[more]