Dataset Information

Post-operative plasma osteopontin predicts distant metastasis in human colorectal cancer.

ABSTRACT:

Background

The overall prognosis of colorectal cancer (CRC) patients is unsatisfactory due to cancer metastasis after operation. This study aims to investigate the clinical significance of plasma osteopontin (OPN) levels as minimally invasive, predictive, and surrogate biomarkers for prognosis of CRC patients.

Methods

This randomized study design consists of pre-operative and post-operative plasma samples from a total of 79 patients. We determined plasma levels of OPN by ELISA and examined their correlation with the clinicopathological parameters of CRC patients. The effects of endogenous and exogenous OPN on CRC metastasis were investigated by examination of the effect on regulators of epithelial to messenchymal transition and migration assay.

Results

Our findings demonstrated for the first time the clinical correlation of plasma OPN with metastasis of CRC patients. High post-operative plasma OPN level (>153.02 ng/ml) associated with development of metastasis after curative resection (p<0.001). Moreover, post-operative plasma OPN level correlated with disease-free survival of CRC patients (p=0.009) and was an independent factor for predicting development of metastasis in CRC patients after curative resection (p=0.036). Our in vitro model showed that OPN ectopic expression induced DLD1 cell migration through Snail and Twist1 overexpression and E-cadherin repression, and secretory OPN level enhanced cell migration.

Conclusions

The results of the current study suggest that post-operative plasma OPN correlated with post-operative metastasis, suggesting that it is a potential non-invasive biomarker for the development of future metastasis in CRC patients. In addition, OPN was shown to be involved in the metastatic process and thus inhibition of OPN is a potential therapeutic approach to treat CRC patients.

SUBMITTER: Ng L

PROVIDER: S-EPMC4427310 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Publications

Post-operative plasma osteopontin predicts distant metastasis in human colorectal cancer.

Ng Lui L Wan Timothy Ming-Hun TM Wan Timothy Ming-Hun TM Lam Colin Siu-Chi CS Chow Ariel Ka-Man AK Wong Sunny Kit-Man SK Man Johnny Hon-Wai JH Li Hung-Sing HS Cheng Nathan Shiu-Man NS Pak Ryan Chung-Hei RC Cheung Alvin Ho-Kwan AH Yau Thomas Chung-Cheung TC Lo Oswens Siu-Hung OS Foo Dominic Chi-Chung DC Poon Jensen Tung-Chung JT Poon Ronnie Tung-Ping RT Pang Roberta Wen-Chi RW Law Wai-Lun WL

PloS one 20150511 5

<h4>Background</h4>The overall prognosis of colorectal cancer (CRC) patients is unsatisfactory due to cancer metastasis after operation. This study aims to investigate the clinical significance of plasma osteopontin (OPN) levels as minimally invasive, predictive, and surrogate biomarkers for prognosis of CRC patients.<h4>Methods</h4>This randomized study design consists of pre-operative and post-operative plasma samples from a total of 79 patients. We determined plasma levels of OPN by ELISA and ...[more]

PMID: 25961724

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Dataset Information

Post-operative plasma osteopontin predicts distant metastasis in human colorectal cancer.

Background

Methods

Results

Conclusions

Publications

Post-operative plasma osteopontin predicts distant metastasis in human colorectal cancer.

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