Ontology highlight
ABSTRACT:
SUBMITTER: Vilanova B
PROVIDER: S-EPMC4428383 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Vilanova Bartolomé B Sanfelice Domenico D Martorell Gabriel G Temussi Piero A PA Pastore Annalisa A
Frontiers in molecular biosciences 20140930
Yfh1, the yeast ortholog of frataxin, is a protein of limited thermodynamic stability which undergoes cold denaturation at temperatures above the water freezing point. We have previously demonstrated that its stability is strongly dependent on ionic strength and that monovalent or divalent cations are able to considerably stabilize the fold. Here, we present a study of the folded state and of the structural determinants that lead to the strong salt dependence. We demonstrate by nuclear magnetic ...[more]