Ontology highlight
ABSTRACT:
SUBMITTER: Kunert B
PROVIDER: S-EPMC4428385 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Kunert Britta B Gardiennet Carole C Lacabanne Denis D Calles-Garcia Daniel D Falson Pierre P Jault Jean-Michel JM Meier Beat H BH Penin François F Böckmann Anja A
Frontiers in molecular biosciences 20140612
We present solid-state NMR sample preparation and first 2D spectra of the Bacillus subtilis ATP-binding cassette (ABC) transporter BmrA, a membrane protein involved in multidrug resistance. The homodimeric 130-kDa protein is a challenge for structural characterization due to its membrane-bound nature, size, inherent flexibility and insolubility. We show that reconstitution of this protein in lipids from Bacillus subtilis at a lipid-protein ratio of 0.5 w/w allows for optimal protein insertion in ...[more]