Ontology highlight
ABSTRACT:
SUBMITTER: Bekesova S
PROVIDER: S-EPMC4429547 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Bekešová Slávka S Komis George G Křenek Pavel P Vyplelová Petra P Ovečka Miroslav M Luptovčiak Ivan I Illés Peter P Kuchařová Anna A Šamaj Jozef J
Frontiers in plant science 20150513
The aim of the present study is to rationalize acrylamide pendant Phos-Tag™ in-gel discrimination of phosphorylated and non-phosphorylated plant protein species with standard immunoblot analysis, and optimize sample preparation, efficient electrophoretic separation and transfer. We tested variants of the method including extraction buffers suitable for preservation of phosphorylated protein species in crude extracts from plants and we addressed the importance of the cation (Mn(2+) or Zn(2+)) use ...[more]