A hybrid-membrane migration method to isolate high-purity adipose-derived stem cells from fat tissues.
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ABSTRACT:
SUBMITTER: Higuchi A
PROVIDER: S-EPMC4429558 | biostudies-literature | 2015 May
REPOSITORIES: biostudies-literature
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A hybrid-membrane migration method to isolate high-purity adipose-derived stem cells from fat tissues.
Higuchi Akon A Wang Ching-Tang CT Ling Qing-Dong QD Lee Henry Hsin-chung HH Kumar S Suresh SS Chang Yung Y Alarfaj Abdullah A AA Munusamy Murugan A MA Hsu Shih-Tien ST Wu Gwo-Jang GJ Umezawa Akihiko A
Scientific reports 20150513
Human adipose-derived stem cells (hADSCs) exhibit heterogeneous characteristics, indicating various genotypes and differentiation abilities. The isolated hADSCs can possess different purity levels and divergent properties depending on the purification methods used. We developed a hybrid-membrane migration method that purifies hADSCs from a fat tissue solution with extremely high purity and pluripotency. A primary fat-tissue solution was permeated through the porous membranes with a pore size fro ...[more]
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