MiR-187 Targets the Androgen-Regulated Gene ALDH1A3 in Prostate Cancer.

ABSTRACT: miRNAs are predicted to control the activity of approximately 60% of all protein-coding genes participating in the regulation of several cellular processes and diseases, including cancer. Recently, we have demonstrated that miR-187 is significantly downregulated in prostate cancer (PCa) and here we propose a proteomic approach to identify its potential targets. For this purpose, PC-3 cells were transiently transfected with miR-187 precursor and miRNA mimic negative control. Proteins were analyzed by a two-dimensional difference gel electrophoresis (2D-DIGE) and defined as differentially regulated if the observed fold change was ±1.06. Then, MALDI-TOF MS analysis was performed after protein digestion and low abundance proteins were identified by LC-MS/MS. Peptides were identified by searchi

SUBMITTER: Casanova-Salas I 

PROVIDER: S-EPMC4430273 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature