Microheterogeneity in frozen protein solutions.
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ABSTRACT:
SUBMITTER: Twomey A
PROVIDER: S-EPMC4430435 | biostudies-literature | 2015 Jun
REPOSITORIES: biostudies-literature
Publications
Microheterogeneity in frozen protein solutions.
Twomey Alan A Kurata Kosaku K Nagare Yutaka Y Takamatsu Hiroshi H Aksan Alptekin A
International journal of pharmaceutics 20150415 1-2
In frozen and lyophilized systems, the biological to be stabilized (e.g. therapeutic protein, biomarker, drug-delivery vesicle) and the cryo-/lyo-protectant should be co-localized for successful stabilization. During freezing and drying, many factors cause physical separation of the biological from the cryo-/lyo-protectant, called microheterogeneity (MH), which may result in poor stabilization efficiency. We have developed a novel technique that utilized confocal Raman microspectroscopy in combi ...[more]
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