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Ammonia stimulates growth and nitrite-oxidizing activity of Nitrobacter winogradskyi.


ABSTRACT: The aim of this study was to obtain a nitrite-oxidizing bacterium with high nitrite oxidation activity for controlling nitrite levels. A nitrite-oxidizing bacterium, ZS-1, was isolated from the water of a coastal Pseudosciaena crocea-rearing pond. The strain was identified as Nitrobacter winogradskyi based on the phylogenetic analyses of the 16S ribosomal ribonucleic acid gene and nxrA sequence of ZS-1. Under aerobic condition, the nitrite-oxidizing activity of ZS-1 did not change considerably in the range of pH 7-9, but was strongly inhibited by lower (pH = 6) and higher (pH = 10) pH values. The optimum temperature range is 25-32 °C. Lower temperature made the adaptive phase of ZS-1 longer but did not affect its maximum nitrite oxidization rate. The nitrite-oxidizing activity of ZS-1 started to be inhibited by ammonia and nitrate when the concentrations of ammonia and nitrate reached 25 mg L-1 and 100 mg L-1, respectively. The inhibition was stronger with higher concentration of ammonia or nitrate. The nitrite-oxidizing activity of ZS-1, however, was not inhibited by high concentration of nitrite (500 mg L-1). The nitrite-oxidizing activity of ZS-1 was increased by low ammonia concentration (1 mg L-1 to 10 mg L-1).

SUBMITTER: Ma S 

PROVIDER: S-EPMC4433873 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature

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Ammonia stimulates growth and nitrite-oxidizing activity of <i>Nitrobacter winogradskyi</i>.

Ma Shouguang S   Zhang Demin D   Zhang Wenjun W   Wang Yinong Y  

Biotechnology, biotechnological equipment 20140101 1


The aim of this study was to obtain a nitrite-oxidizing bacterium with high nitrite oxidation activity for controlling nitrite levels. A nitrite-oxidizing bacterium, ZS-1, was isolated from the water of a coastal <i>Pseudosciaena crocea</i>-rearing pond. The strain was identified as <i>Nitrobacter winogradskyi</i> based on the phylogenetic analyses of the 16S ribosomal ribonucleic acid gene and <i>nxrA</i> sequence of ZS-1. Under aerobic condition, the nitrite-oxidizing activity of ZS-1 did not  ...[more]

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