Ontology highlight
ABSTRACT:
SUBMITTER: Ouyang K
PROVIDER: S-EPMC4434054 | biostudies-literature | 2014 Nov
REPOSITORIES: biostudies-literature
Biotechnology, biotechnological equipment 20141121 6
Plant tissues contain abundant polysaccharides, phenolic compounds and other metabolites, which makes it difficult to isolate high-quality RNA from them. In addition, <i>Neolamarckia cadamba</i> contains large quantities of other components, particularly RNA-binding alkaloids, which makes the isolation even more challenging. Here, we describe a concise and efficient RNA isolation method that combines the cetyltrimethyl ammonium bromide (CTAB) and Plant RNA Kit (Omega) protocols. Gel electrophore ...[more]