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Intermonomer electron transfer between the b hemes of heterodimeric cytochrome bc(1).

ABSTRACT: The ubihydroquinone:cytochrome c oxidoreductase, or cytochrome bc1, is a central component of respiratory and photosynthetic energy transduction pathways in many organisms. It contributes to the generation of membrane potential and proton gradient used for cellular energy (ATP) production. The three-dimensional structures of cytochrome bc1 show a homodimeric organization of its three catalytic subunits. The unusual architecture revived the issue of whether the monomers operate independently or function cooperatively during the catalytic cycle of the enzyme. In recent years, different genetic approaches allowed the successful production of heterodimeric cytochrome bc1 variants and evidenced the occurrence of intermonomer electron transfer between the monomers of this enzyme. Here we used a version of the "two-plasmid" genetic system, also described in the preceding paper (DOI: 10.1021/bi400560p), to study a new heterodimeric mutant variant of cytochrome bc1. The strain producing this heterodimeric variant sustained photosynthetic growth of Rhodobacter capsulatus and yielded an active heterodimer. Interestingly, kinetic data showed equilibration of electrons among the four b heme cofactors of the heterodimer, via "reverse" intermonomer electron transfer between the bL hemes. Both inactive homodimeric and active heterodimeric cytochrome bc1 variants were purified to homogeneity from the same cells, and purified samples were subjected to mass spectrometry analyses. The data unequivocally supported the idea that the cytochrome b subunits carried the expected mutations and their associated epitope tags. Implications of these findings on our interpretation of light-activated transient cytochrome b and c redox kinetics and the mechanism of function of a dimeric cytochrome bc1 are discussed with respect to the previously proposed heterodimeric Q cycle model.

SUBMITTER: Lanciano P

PROVIDER: S-EPMC4436693 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature

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Intermonomer electron transfer between the b hemes of heterodimeric cytochrome bc(1).

Lanciano Pascal P Khalfaoui-Hassani Bahia B Selamoglu Nur N Daldal Fevzi F

Biochemistry 20131001 41

The ubihydroquinone:cytochrome c oxidoreductase, or cytochrome bc1, is a central component of respiratory and photosynthetic energy transduction pathways in many organisms. It contributes to the generation of membrane potential and proton gradient used for cellular energy (ATP) production. The three-dimensional structures of cytochrome bc1 show a homodimeric organization of its three catalytic subunits. The unusual architecture revived the issue of whether the monomers operate independently or f ...[more]

PMID: 24028549

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Project description:Cytochrome c(1) of Rhodobacter (Rba.) species provides a series of mutants which change barriers for electron transfer through the cofactor chains of cytochrome bc(1) by modifying heme c(1) redox midpoint potential. Analysis of post-flash electron distribution in such systems can provide useful information about the contribution of individual reactions to the overall electron flow. In Rba. capsulatus, the non-functional low-potential forms of cytochrome c(1) which are devoid of the disulfide bond naturally present in this protein revert spontaneously by introducing a second-site suppression (mutation A181T) that brings the potential of heme c(1) back to the functionally high levels, yet maintains it some 100 mV lower from the native value. Here we report that the disulfide and the mutation A181T can coexist in one protein but the mutation exerts a dominant effect on the redox properties of heme c(1) and the potential remains at the same lower value as in the disulfide-free form. This establishes effective means to modify a barrier for electron transfer between the FeS cluster and heme c(1) without breaking disulfide. A comparison of the flash-induced electron transfers in native and mutated cytochrome bc(1) revealed significant differences in the post-flash equilibrium distribution of electrons only when the connection of the chains with the quinone pool was interrupted at the level of either of the catalytic sites by the use of specific inhibitors, antimycin or myxothiazol. In the non-inhibited system no such differences were observed. We explain the results using a kinetic model in which a shift in the equilibrium of one reaction influences the equilibrium of all remaining reactions in the cofactor chains. It follows a rather simple description in which the direction of electron flow through the coupled chains of cytochrome bc(1) exclusively depends on the rates of all reversible partial reactions, including the Q/QH2 exchange rate to/from the catalytic sites.

Dataset Information

Intermonomer electron transfer between the b hemes of heterodimeric cytochrome bc(1).

Publications

Intermonomer electron transfer between the b hemes of heterodimeric cytochrome bc(1).

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