Unknown

Dataset Information

0

LEF1 Targeting EMT in Prostate Cancer Invasion Is Regulated by miR-34a.


ABSTRACT: The microRNA-34a (miR-34a), a tumor-suppressive microRNA (miRNA), is implicated in epithelial-mesenchymal transition (EMT) and cancer stem cells. Lymphoid enhancer-binding factor-1 (LEF1) is a key transcription factor in the Wnt signaling pathway, and has been suggested to be involved in regulation of cell proliferation and invasion. Here, the molecular mechanism of miR-34a and LEF1 in cooperatively regulating prostate cancer cell invasion is described. Molecular profiling analysis of miRNA levels in prostate cancer cells revealed a negative correlation between miR-34a and LEF1 expression, and the downregulation of LEF1 by miR-34a was confirmed by luciferase assays. Furthermore, miR-34a specifically repressed LEF1 expression through direct binding to its 3'-untranslated regions (3'-UTR). miR-34a modulated the levels of LEF1 to regulate EMT in prostate cancer cells. Functionally, miR-34a negatively correlated with the migration and invasion of prostate cancer cells through LEF1. An analysis of miR-34a expression levels in matched human tumor and benign tissues demonstrated consistent and statistically significant downregulation of miR-34a in primary prostate cancer specimens. These data strongly suggest that miR-34a/LEF1 regulation of EMT plays an important role in prostate cancer migration and invasion.The miR-34a-LEF1 axis represents a potential molecular target for novel therapeutic strategies in prostate cancer.

SUBMITTER: Liang J 

PROVIDER: S-EPMC4437214 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature

altmetric image

Publications

LEF1 Targeting EMT in Prostate Cancer Invasion Is Regulated by miR-34a.

Liang Jiaqian J   Li Yirong Y   Daniels Garrett G   Sfanos Karen K   De Marzo Angelo A   Wei Jianjun J   Li Xin X   Chen Wenqiang W   Wang Jinhua J   Zhong Xuelin X   Melamed Jonathan J   Zhao Jun J   Lee Peng P  

Molecular cancer research : MCR 20150113 4


<h4>Unlabelled</h4>The microRNA-34a (miR-34a), a tumor-suppressive microRNA (miRNA), is implicated in epithelial-mesenchymal transition (EMT) and cancer stem cells. Lymphoid enhancer-binding factor-1 (LEF1) is a key transcription factor in the Wnt signaling pathway, and has been suggested to be involved in regulation of cell proliferation and invasion. Here, the molecular mechanism of miR-34a and LEF1 in cooperatively regulating prostate cancer cell invasion is described. Molecular profiling ana  ...[more]

Similar Datasets

| S-EPMC3973098 | biostudies-literature
| S-EPMC7574442 | biostudies-literature
| S-EPMC4674136 | biostudies-literature
| S-EPMC3731248 | biostudies-literature
| S-EPMC7579717 | biostudies-literature
| S-EPMC9818453 | biostudies-literature
| S-EPMC8961520 | biostudies-literature
| S-EPMC5595080 | biostudies-literature
| S-EPMC7061746 | biostudies-literature
| S-EPMC5596596 | biostudies-literature