Pharmacological methyl group donors block skeletal metastasis in vitro and in vivo.

ABSTRACT:

Background and purpose

DNA hypomethylation was previously implicated in metastasis. In the present study, we examined whether methyl supplementation with the universal methyl donor S-adenosylmethionine (SAM) inhibits prostate cancer associated skeletal metastasis.

Experimental approach

Highly invasive human prostate cancer cells PC-3 and DU-145 were treated with vehicle alone, S-adenosylhomocysteine (SAH) or SAM and their effects on tumour cell proliferation, invasion, migration and colony formation were monitored. For in vivo studies, control (SAH) and SAM-treated PC-3 cells were injected into the tibia of Fox chase SCID mice and skeletal lesions were determined by X-ray and μCT. To understand possible mechanisms involved, we delineated the effect of SAM on the genome-wide

SUBMITTER: Shukeir N 

PROVIDER: S-EPMC4439874 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature