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Molecular cloning and sequencing analysis of the interferon ? from Coturnix.

ABSTRACT: One pair of primers was designed according to Gallus and Meleagris gallopavo interferon ? (IFN-?) sequences published in GenBank. The primers and RNA extraction from the spleen of Coturnix were used to amplify Coturnix IFN-? cDNA by real-time polymerase chain reaction (RT-PCR). The product was cloned into pEasy-T1 vector. Evaluating recombinant plasmid by PCR and restriction enzyme digestion. Sequence the cloning sequences, comparing the sequencing results by NCBI. We successfully got a Coturnix IFN-? partial sequence. The sequence was subtyped and put to homologous analysis. The results suggested the homology of IFN-? gene of Coturnix and gene of Coturnix and chicken (88.7%), the homology of IFN-? gene of Coturnix and chicken (88.7%), the homology of IFN-? gene of Coturnix and Anas platyrhynchos (72.5%), the homology of IFN-? sequence registered in GenBank. The analysis of the genetic tree showed that the relationship of Coturnix and chicken IFN-? had a high homology. It can be seen that in this study we successfully got a partial sequence of IFN-? of quail.

SUBMITTER: Zheng B 

PROVIDER: S-EPMC4439982 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Molecular cloning and sequencing analysis of the interferon β from Coturnix.

Zheng Bei B   Chang Wei-Shan WS  

Central-European journal of immunology 20140417 1

One pair of primers was designed according to Gallus and Meleagris gallopavo interferon β (IFN-β) sequences published in GenBank. The primers and RNA extraction from the spleen of Coturnix were used to amplify Coturnix IFN-β cDNA by real-time polymerase chain reaction (RT-PCR). The product was cloned into pEasy-T1 vector. Evaluating recombinant plasmid by PCR and restriction enzyme digestion. Sequence the cloning sequences, comparing the sequencing results by NCBI. We successfully got a Coturnix  ...[more]

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