Dataset Information

Detection of Peptide-based nanoparticles in blood plasma by ELISA.

ABSTRACT:

Aims

The aim of the current study was to develop a method to detect peptide-linked nanoparticles in blood plasma.

Materials & methods

A convenient enzyme linked immunosorbent assay (ELISA) was developed for the detection of peptides functionalized with biotin and fluorescein groups. As a proof of principle, polymerized pentafluorophenyl methacrylate nanoparticles linked to biotin-carboxyfluorescein labeled peptides were intravenously injected in Wistar rats. Serial blood plasma samples were analyzed by ELISA and by liquid chromatography mass spectrometry (LC/MS) technology.

Results

The ELISA based method for the detection of FITC labeled peptides had a detection limit of 1 ng/mL. We were able to accurately measure peptides bound to pentafluorophenyl methacrylate nanoparticles in blood plasma of rats, and similar results were obtained by LC/MS.

Conclusions

We detected FITC-labeled peptides on pentafluorophenyl methacrylate nanoparticles after injection in vivo. This method can be extended to detect nanoparticles with different chemical compositions.

SUBMITTER: Bode GH

PROVIDER: S-EPMC4440766 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Publications

Detection of Peptide-based nanoparticles in blood plasma by ELISA.

Bode Gerard H GH Pickl Karin E KE Sanchez-Purrà Maria M Albaiges Berta B Borrós Salvador S Pötgens Andy J G AJ Schmitz Christoph C Sinner Frank M FM Losen Mario M Steinbusch Harry W M HW Frank Hans-Georg HG Martinez-Martinez Pilar P

PloS one 20150521 5

<h4>Aims</h4>The aim of the current study was to develop a method to detect peptide-linked nanoparticles in blood plasma.<h4>Materials & methods</h4>A convenient enzyme linked immunosorbent assay (ELISA) was developed for the detection of peptides functionalized with biotin and fluorescein groups. As a proof of principle, polymerized pentafluorophenyl methacrylate nanoparticles linked to biotin-carboxyfluorescein labeled peptides were intravenously injected in Wistar rats. Serial blood plasma sa ...[more]

PMID: 25996618

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Dataset Information

Detection of Peptide-based nanoparticles in blood plasma by ELISA.

Aims

Materials & methods

Results

Conclusions

Publications

Detection of Peptide-based nanoparticles in blood plasma by ELISA.

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