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Taming molecular flexibility to tackle rare diseases.

ABSTRACT: Many mutations responsible of Fabry disease destabilize lysosomal alpha-galactosidase, but retain the enzymatic activity. These mutations are associated to a milder phenotype and are potentially curable with a pharmacological therapy either with chaperones or with drugs that modulate proteostasis. We demonstrate the effectiveness of molecular dynamics simulations to correlate the genotype to the severity of the disease. We studied the relation between protein flexibility and residual enzymatic activity of pathological missense mutants in the cell. We found that mutations occurring at flexible sites are likely to retain activity in vivo. The usefulness of molecular dynamics for diagnostic purposes is not limited to lysosomal galactosidase because destabilizing mutations are widely encountered in other proteins, too, and represent a large share of all the ones associated to human diseases.

SUBMITTER: Cubellis MV

PROVIDER: S-EPMC4441037 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature

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Taming molecular flexibility to tackle rare diseases.

Cubellis Maria Vittoria MV Baaden Marc M Andreotti Giuseppina G

Biochimie 20150402

Many mutations responsible of Fabry disease destabilize lysosomal alpha-galactosidase, but retain the enzymatic activity. These mutations are associated to a milder phenotype and are potentially curable with a pharmacological therapy either with chaperones or with drugs that modulate proteostasis. We demonstrate the effectiveness of molecular dynamics simulations to correlate the genotype to the severity of the disease. We studied the relation between protein flexibility and residual enzymatic a ...[more]

PMID: 25841341

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Taming molecular flexibility to tackle rare diseases.

Publications

Taming molecular flexibility to tackle rare diseases.

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