Ontology highlight
ABSTRACT:
SUBMITTER: Kulinski MD
PROVIDER: S-EPMC4442481 | biostudies-literature | 2009 Jun
REPOSITORIES: biostudies-literature
Kulinski M Dominika MD Mahalanabis Madhumita M Gillers Sara S Zhang Jane Y JY Singh Satish S Klapperich Catherine M CM
Biomedical microdevices 20090601 3
Silica impregnated polymer monolithic columns may provide a simple method for lysing and extracting DNA from bacteria inside of microfluidic chips. Here we use Escherichia coli as a test organism for a point of care thermoplastic microfluidic module designed to take in a urine sample, mix it with lysis buffer, and perform a hybrid chemical/mechanical lysis and solid phase extraction of nucleic acids from the sample. To demonstrate proof-of-concept, we doped human hematuric urine samples with E. ...[more]