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Optimization studies for enhanced bacteriocin production by Pediococcus pentosaceus KC692718 using response surface methodology.

ABSTRACT: Bacteriocins have been produced by various Lactic acid bacteria (LAB) strains isolated from dairy and fermented vegetable sources. In the current study we have isolated a novel bacteriocin producing strain Pediococcus pentosaceus KC692718 from mixed vegetable pickles (India). A 2 step process optimization for enhancing production of bacteriocin from the isolates was carried out with One-factor-at-a-time (OFAT) and Response Surface Methodology (RSM) methods. A 2.5 fold (AU/mL) increase of bacteriocin was observed for sucrose (2.4 %) as carbon source and 4.7 fold (AU/mL) increased bacteriocin was observed in the presence of soyatone (1.03 %) as nitrogen source in the OFAT experiments. In order to increase bacteriocin production RSM tool was performed with optimized chemical and physical sources using Design expert 8.0.7.1. Soyatone (1.03 %), sucrose (2.4 %), pH (5.5) and temperature (34.5?ºC) condition yielded 25,600.34 AU/mL of bacteriocin from P. pentosaceus KC692718. This is the first report which has produced 20 fold increase of bacteriocin for Pediococcus pentosaceus KC692718 from that of MRS medium with 1?280 AU/mL.

SUBMITTER: Suganthi V

PROVIDER: S-EPMC4444917 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature

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Optimization studies for enhanced bacteriocin production by Pediococcus pentosaceus KC692718 using response surface methodology.

Suganthi V V Mohanasrinivasan V V

Journal of food science and technology 20140616 6

Bacteriocins have been produced by various Lactic acid bacteria (LAB) strains isolated from dairy and fermented vegetable sources. In the current study we have isolated a novel bacteriocin producing strain Pediococcus pentosaceus KC692718 from mixed vegetable pickles (India). A 2 step process optimization for enhancing production of bacteriocin from the isolates was carried out with One-factor-at-a-time (OFAT) and Response Surface Methodology (RSM) methods. A 2.5 fold (AU/mL) increase of bacteri ...[more]

PMID: 26028762

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Project description:IntroductionA halophilic bacterium of the Halomonas elongata BK-AG25 has successfully produced ectoine with high productivity. To overcome the drawbacks of high levels of salt in the production process, a nonhalophilic bacteria of Escherichia coli (E. coli) was used to express the ectoine gene cluster of the halophilic bacteria, and the production of ectoine by the recombinant cell was optimized.MethodsThe ectoine gene cluster from the halophilic bacterium was isolated and inserted into an expression plasmid of pET30(a) and subsequently transformed into E. coli BL21 (DE3). Production of ectoine from the recombinant E. coli was investigated and then maximized by optimizing the level of nutrients in the medium, as well as the bioprocess conditions using response surface methodology. The experimental designs were performed using a central composite design.ResultsThe recombinant E. coli successfully expressed the ectoine gene cluster of Halomonas elongata BK-AG25 under the control of the T7 promoter. The recombinant cell was able to produce ectoine, of which most were excreted into the medium. The optimization of ectoine production with the response surface methodology showed that the level of salt in the medium, the incubation temperature, the optical density of the bacteria before induction, and the final concentration of the inducer gave a significant effect on ectoine production by the recombinant E. coli. Interestingly, the level of salt in the medium and the incubation temperature showed an inverse effect on the production of intracellular and extracellular ectoine by the recombinant cell. At the optimum conditions, the production yield was about 418 mg ectoine/g cdw (cell dry weight) after 12 hours of incubation.ConclusionThis study is the first report on the expression of an ectoine gene cluster of Halomonas elongata BK-AG25 in E. coli BL21, under the control of the T7 promoter. Optimization of the level of nutrients in the medium, as well as the bioprocess condition using response surface methodology, has successfully increased the production of ectoine by the recombinant bacteria.

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Optimization studies for enhanced bacteriocin production by Pediococcus pentosaceus KC692718 using response surface methodology.

Publications

Optimization studies for enhanced bacteriocin production by Pediococcus pentosaceus KC692718 using response surface methodology.

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