Unknown

Dataset Information

0

Single nucleotide polymorphisms in the bovine Histophilus somni genome; a comparison of new and old isolates.


ABSTRACT: Histophilus somni, a causative agent of the bovine respiratory disease complex, can also cause a variety of systemic disorders, including bronchopneumonia, myocarditis, pericarditis, arthritis, pleuritis, and infectious thrombotic meningoencephalitis. The purpose of this study was to determine if currently circulating strains differ from those of the 1980s by identifying genomic changes. Single nucleotide polymorphisms (SNPs) and insertion and deletion (INDEL) sites were examined by whole-genome sequencing in 12 samples, 6 old and 6 new. The 31 028 SNP/INDELs recorded were compared against the reference genome sequence of the pathogenic H. somni strain 2336. The distribution of about 75% of these SNPs within a specified gene differed between old and new isolates and did not follow any particular pattern. The other 25% clustered into 2 groups containing the same SNPs in various genes: group I included 5 old isolates and 1 new isolate; group II included 5 new isolates and 1 old isolate. For putative virulence genes there were more SNPs in group I compared with strain 2336, itself an older isolate, than in group II. Although only 25% of all the SNPs formed 2 clusters, the results suggest some genetic difference in various genes between old and new strains.

SUBMITTER: Madampage CA 

PROVIDER: S-EPMC4445511 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature

altmetric image

Publications

Single nucleotide polymorphisms in the bovine Histophilus somni genome; a comparison of new and old isolates.

Madampage Claudia Avis CA   Rawlyk Neil N   Crockford Gordon G   Van Donkersgoed Joyce J   Dorin Craig C   Potter Andrew A  

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire 20150701 3


Histophilus somni, a causative agent of the bovine respiratory disease complex, can also cause a variety of systemic disorders, including bronchopneumonia, myocarditis, pericarditis, arthritis, pleuritis, and infectious thrombotic meningoencephalitis. The purpose of this study was to determine if currently circulating strains differ from those of the 1980s by identifying genomic changes. Single nucleotide polymorphisms (SNPs) and insertion and deletion (INDEL) sites were examined by whole-genome  ...[more]

Similar Datasets

| S-EPMC5629063 | biostudies-literature
| S-EPMC5565109 | biostudies-other
| S-EPMC3262788 | biostudies-literature
2011-10-01 | GSE29578 | GEO
| S-EPMC4747540 | biostudies-literature
| S-EPMC8487530 | biostudies-literature
2011-09-30 | E-GEOD-29578 | biostudies-arrayexpress
| S-EPMC7088796 | biostudies-literature
| S-EPMC6066786 | biostudies-literature
| S-EPMC8059806 | biostudies-literature