Using RecA protein to enhance kinetic rates of DNA circuits.
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ABSTRACT:
SUBMITTER: Milligan JN
PROVIDER: S-EPMC4446201 | biostudies-literature | 2015 Jun
REPOSITORIES: biostudies-literature
Publications
Using RecA protein to enhance kinetic rates of DNA circuits.
Milligan J N JN Ellington A D AD
Chemical communications (Cambridge, England) 20150601 46
While DNA circuits are becoming increasingly useful as signal transducers, their utility is inhibited by their slow catalytic rate. Here, we demonstrate how RecA, a recombination enzyme that catalyzes sequence specific strand exchange, can be used to increase circuit rates up to 9-fold. We also show how the introduction of RNA into DNA circuits further controls the specificity of RecA strand exchange, improving signal-to-noise. ...[more]
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