Ontology highlight
ABSTRACT:
SUBMITTER: Chen H
PROVIDER: S-EPMC4446711 | biostudies-literature | 2015 Mar
REPOSITORIES: biostudies-literature
Chen Hu H Yuan Guohua G Winardhi Ricksen S RS Yao Mingxi M Popa Ionel I Fernandez Julio M JM Yan Jie J
Journal of the American Chemical Society 20150309 10
The mechanical stability of force-bearing proteins is crucial for their functions. However, slow transition rates of complex protein domains have made it challenging to investigate their equilibrium force-dependent structural transitions. Using ultra stable magnetic tweezers, we report the first equilibrium single-molecule force manipulation study of the classic titin I27 immunoglobulin domain. We found that individual I27 in a tandem repeat unfold/fold independently. We obtained the force-depen ...[more]