Ontology highlight
ABSTRACT:
SUBMITTER: Mae SI
PROVIDER: S-EPMC4447148 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Mae Shin-Ichi SI Shono Akemi A Shiota Fumihiko F Yasuno Tetsuhiko T Kajiwara Masatoshi M Gotoda-Nishimura Nanaka N Arai Sayaka S Sato-Otubo Aiko A Toyoda Taro T Takahashi Kazutoshi K Nakayama Naoki N Cowan Chad A CA Aoi Takashi T Ogawa Seishi S McMahon Andrew P AP Yamanaka Shinya S Osafune Kenji K
Nature communications 20130101
A method for stimulating the differentiation of human pluripotent stem cells into kidney lineages remains to be developed. Most cells in kidney are derived from an embryonic germ layer known as intermediate mesoderm. Here we show the establishment of an efficient system of homologous recombination in human pluripotent stem cells by means of bacterial artificial chromosome-based vectors and single-nucleotide polymorphism array-based detection. This system allowed us to generate human-induced plur ...[more]